![]() ![]() Nematode characterization was carried out based on morphological information obtained from light microscopy (LM) and scanning electron microscopy (SEM) studies. The potential importance of this nematode as a pest of finger millet was evidenced by its dense population in the soil sample and its presence observed in the finger millet root system (revealed by the fuchsin staining method). found associated with finger millet, Eleusine coracana (L.) Gaertn. In the current paper, we characterize a newly discovered Rotylenchus wimbii n. ![]() In combination with the morphological features, molecular information such as ITS, 18S, and 28S of ribosomal DNA and COI of mitochondrial DNA have been used for their identification. This identification system has so far been widely accepted and its widespread use has also recently been facilitated by a web-based key that draws its basis from cluster analysis ( Nguyen et al., 2019). ![]() To facilitate the identification of Rotylenchus species, Castillo and Vovlas (2005) have developed a tabular or matrix key using a total of 11 characters of this nematode group. As obligate root parasites feeding on root hairs or epidermal or cortical cells of a plant, they can cause prominent damage to the host plant, with symptoms ranging from stunted and reduced growth to wilting, chlorosis, reduced root system and root lesions, often leading to significant losses in crop yield ( Castillo and Vovlas, 2005 Manzanilla López and Marbán Mendoza, 2012 Sikora et al., 2018). have been reported from all the continents, being found associated with numerous important crops ranging from rice and cereals to tubers, vegetables, and even ornamental plants and trees. Within the plant-parasitic nematode (PPN) genus Rotylenchus Filipjev, 1936 (Nematoda: Hoplolaimidae), 104 valid species have been recognized ( Nguyen et al., 2019). and revealed some mislabeled Rotylenchus brevicaudatus sequences in GenBank. Molecular phylogenies, in combination with species delimitation, supported the distinctiveness of Rotylenchus wimbii n. This new species is characterized by a moderate female body size of 0.6 to 0.8 mm, a continuous hemispherical lip region with four annuli, 3 to 4 irregular blocks on the basal lip annule, absence of longitudinal cuticular striations in anterior region, four lateral lines forming three equal bands which are areolated mainly at pharynx level, a robust stylet of 23 to 27 µm of which 45 to 53% is cone part, and with rounded to sometimes indented knobs, a secretory-excretory pore around level of pharyngo-intestinal junction, didelphic-amphidelphic reproductive system, vulva without distinct epiptygma, indistinct to empty spermatheca, tail usually truncated with 5 to 9 annuli, phasmids located at 7 to 17 annuli anterior to anus, and absence of males. Sequence analysis was performed on ITS, 18S, and D2-D3 of 28S of ribosomal DNA and COI of mitochondrial DNA. was found associated with finger millet in Kenya and is described based on light microscopy, scanning electron microscopy, and molecular information.
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